T5450

Human Primary Prostate Microvascular Endothelial Cells

1594.29 €

ABC-TC4199

Rat Prostate Microvascular Endothelial Cells

Rat Prostate Microvascular Endothelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal Sprague-Dawley Rats. Rat Prostate Microvascular Endothelial Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.Rat Prostate Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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ABC-TC3766

Human Prostate Microvascular Endothelial Cells

Primary Human Prostate Microvascular Endothelial Cells were initiated by elutriation of dispase dissociated normal prostate tissue.These cells were originated using Complete Serum-Free Medium Kit With AcceSup™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

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ABC-TC3524

Human Brain Microvascular Endothelial Cells

Human Brain Microvascular Endothelial Cells were initiated by elutriation of dispase dissociated normal human brain cortex tissue.These cells were originated using Complete Serum-Free Medium Kit, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells.

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ABC-TC3540

Human Cardiac Microvascular Endothelial Cells

The endothelial cells modulate vascular tone by release of several endothelium-derived contracting and relaxing factors, by regulation and degradation of vasoactive peptides, and by enzymes located on the endothelial surface. These functions vary within different spaces, within blood vessels of different organs, and between the macro- and microvasculations. Cardiac microvascular endothelial cells (CMEC) exert physiological and pathophysiological effects on the function of cardiac myocytes. They release cardioactive substances including nitric oxide, endothelin-1, prostanoids, adenylpurines, natriuretic peptides, and other agents, that modify and regulate cardiac myocyte function. Such effects of endothelial cells have been demonstrated both in vivo and in vitro. CMEC also possess enzymatic activities, in particular ACE/kininase activity, which can alter local levels of angiotensin II and bradykinin in the heart. HCMEC from Gentaur Research Laboratories are isolated from human heart tissue. HCMEC are cryopreserved after purification and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HCMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCMEC are guaranteed to further expand for 15 population doublings at the conditions provided by Gentaur Research Laboratories.

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ABC-TC3571

Human Colonic Microvascular Endothelial Cells

Endothelial cells lining the microvasculature are known to play a critical "gatekeeper" role in the inflammatory process through their ability to recruit circulating immune cells into tissues and foci of inflammation. Studies show that intestinal microvascular endothelial cells exhibit a strong immune response to LPS challenge and play a critical regulatory role in gut inflammation. Pharmacological inhibition of NOS in activated intestinal microvascular endothelial cells resulted in a significant increase in leukocytes binding. Gene expression profile study reveals that intestinal endothelial cells express biotinidase, which is involved in biotin recycling. The in vitro culture of these cells enabled scientists to perform systematic analyses of the cytokine profiles with regard to mRNA expression and protein secretion, and to compare such data with cytokine profiles concomitantly displayed by other endothelial cells.HCoMEC from Gentaur Research Laboratories are isolated from human colonic tissue. HCoMEC are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCoMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HCoMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCoMEC are guaranteed to further culture in the conditions provided by Gentaur Research Laboratories.

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