T5405

Human Primary Colonic Microvascular Endothelial Cells

1594.29 €

ABC-TC3571

Human Colonic Microvascular Endothelial Cells

Endothelial cells lining the microvasculature are known to play a critical "gatekeeper" role in the inflammatory process through their ability to recruit circulating immune cells into tissues and foci of inflammation. Studies show that intestinal microvascular endothelial cells exhibit a strong immune response to LPS challenge and play a critical regulatory role in gut inflammation. Pharmacological inhibition of NOS in activated intestinal microvascular endothelial cells resulted in a significant increase in leukocytes binding. Gene expression profile study reveals that intestinal endothelial cells express biotinidase, which is involved in biotin recycling. The in vitro culture of these cells enabled scientists to perform systematic analyses of the cytokine profiles with regard to mRNA expression and protein secretion, and to compare such data with cytokine profiles concomitantly displayed by other endothelial cells.HCoMEC from Gentaur Research Laboratories are isolated from human colonic tissue. HCoMEC are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCoMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HCoMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCoMEC are guaranteed to further culture in the conditions provided by Gentaur Research Laboratories.

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ABC-TC5477

BKS DB Mouse Colonic Microvascular Endothelial Cells

BKS db Mouse Colonic Microvascular Endothelial Cells are isolated from the colonic microvascular of Mice homozygous for the diabetes spontaneous mutation (Lepr/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic. BKS db Mouse Colonic Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibod

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ABC-TC3594

Human Dermal Microvascular Endothelial Cells

Blood vessels of the skin are composed of a number of cellular and non-cellular elements. The predominant cell type in all skin blood vessels is the endothelial cell. Endothelial cells form the interface between intravascular and extravascular compartments and serve as a selective barrier for the diffusion of cells and macromolecules between these two compartments. The endothelial cells lining blood vessels of the skin share features of endothelial cells elsewhere in the body, but they also exhibit several skin specific characteristics. They are active participants in a variety of physiological processes. These include wound healing, control of hemostasis, temperature regulation, and the modulation of inflammation/leukocyte trafficking. The structure and function of endothelial cells are differentially regulated in space and time. Endothelial cell proliferation, quiescence, apoptosis, and senescence are the four principal cytologic states that set the cutaneous microvascular in a dynamic balance between maintenance and remodeling.HDMEC from Gentaur Research Laboratories are isolated from human dermal tissue. HDMEC are cryopreserved on passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HDMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HDMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HDMEC are guaranteed to further expand for 15 population doublings in the conditions provided by Gentaur Research Laboratories.

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ABC-TC3614

Human Esophageal Microvascular Endothelial Cells

Endothelial cells lining the microvasculature are known to play a critical gatekeeper role in the inflammatory process through their ability to recruit circulating immune cells into tissues and foci of inflammation.

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ABC-TC3631

Human Glomerular Microvascular Endothelial Cells

Primary Human Glomerular Microvascular Endothelial Cells were initiated by decapsulated glomeruli isolated from normal human kidney cortical tissue.These cells were originated using Complete Serum-Free Medium Kit With AcceSup™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

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