CSC-C4985J

Human Hypertension Cardiac Microvascular Endothelial Cells

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Ab1000

Rat Cardiac Microvascular Endothelial Cells

404.30 €

ABC-TC3540

Human Cardiac Microvascular Endothelial Cells

The endothelial cells modulate vascular tone by release of several endothelium-derived contracting and relaxing factors, by regulation and degradation of vasoactive peptides, and by enzymes located on the endothelial surface. These functions vary within different spaces, within blood vessels of different organs, and between the macro- and microvasculations. Cardiac microvascular endothelial cells (CMEC) exert physiological and pathophysiological effects on the function of cardiac myocytes. They release cardioactive substances including nitric oxide, endothelin-1, prostanoids, adenylpurines, natriuretic peptides, and other agents, that modify and regulate cardiac myocyte function. Such effects of endothelial cells have been demonstrated both in vivo and in vitro. CMEC also possess enzymatic activities, in particular ACE/kininase activity, which can alter local levels of angiotensin II and bradykinin in the heart. HCMEC from Gentaur Research Laboratories are isolated from human heart tissue. HCMEC are cryopreserved after purification and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HCMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCMEC are guaranteed to further expand for 15 population doublings at the conditions provided by Gentaur Research Laboratories.

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ABC-TC3864

Human Uterine Microvascular Endothelial Cells

The walls of capillaries are composed of a single layer of microvascular endothelial cells. These cells differ in morphology and other properties depending on the tissues the capillaries supply. Therefore, Gentaur offers a range of Microvascular Endothelial Cells produced at Gentaur's cell culture facility from normal human tissues of different origins.Shortly after isolation, all Gentaur Microvascular Endothelial Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

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ABC-TC4028

Porcine Brain Microvascular Endothelial Cells

Porcine Brain Microvascular Endothelial Cells are derived from healthly porcinine Brain tissue. Porcine Brain Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.Porcine Brain Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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ABC-TC4081

Rat Bladder Microvascular Endothelial Cells

Rat Bladder Microvascular Endothelial Cells from Gentaur are isolated from tissue of 7-day-old neonatal Sprague-Dawley Rats. Rat Bladder Microvascular Endothelial Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.Rat Bladder Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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