Mouse Hepatic Sinusoidal Endothelial Cells (MHSEC) are isolated from Swiss mice. MHSEC are packaged immediately after purification and delivered as fresh suspensions (0o and +8 centigrade.) ready to use. MHSEC are not recommend for expanding or long term cultures.
Rat hepatic sinusoidal endothelial cells are isolated from tissue of 1-day-old neonatal Sprague-Dawley rats. Rat hepatic sinusoidal endothelial cells are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.