Catalog number: 566 - CSC-7783W
Product Category: Business & Industrial > Science & Laboratory
Size: One Frozen vial
ABC-TC3612
The human esophagus is lined by a non-keratinizing, moist stratified squamous epithelium whose apical cell membranes and intercellular junctional complexes combine to produce an effective permeability barrier against the influx of luminal content. In particular, the barrier created by these structures limits exposure of the surface cells' basolateral cell membranes and entire membrane of cells of the deeper layers to the wide swings in osmolality occurring regularly within the esophageal lumen. Histologically, the esophageal epithelium consists of two zones, the basal and differentiated zones. Cellular proliferation is limited to the basal zone, and cells are thought to migrate from this area towards the esophageal lumen. Migration is associated with the initiation of differentiation and the sequential expression of differentiation markers. The availability of human esophageal epithelial cell culture provides an excellent in vitro model in the study of the physiology of esophageal epithelium and the mechanisms of the esophageal carcinogenesis.HEEC from Gentaur Research Laboratories are isolated from the human esophagus. HEEC are cryopreserved on passage one culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HEEC are characterized by immunofluorescent method with antibodies to cytokeratine-8, -18 and -19. HEEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEEC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.
ABC-TC4133
Rat Primary Esophageal Epithelial Cells from Gentaur are isolated from tissue of adult laboratory Sprague–Dawley Rats. Rat Primary Esophageal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Primary Esophageal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Primary Esophageal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
ABC-TC4601
Proliferation and Morphology: Normal morphology for 5 population doublings, minimum 70% viability when thawed from cryopreservation. Normal Human Endometrial (Uterine) Epithelial Cells provide an ideal serum-free culture model for many areas of research. These cells may be used to study cellular physiology of the reproductive tract, the cellular response to infectious agents, and other areas of research, Endometrial Epithelial Cells have been isolated, plated, and expanded in culture vessels three times before being harvested for cryopreservation to ensure the highest purity, viability and plating efficiency.
ABC-TC5466
Cervical Epithelial Cells have been isolated, plated, and expanded in culture vessels three times before being harvested for cryopreserved to ensure the highest purity, viability and plating efficiency. HCxEC are quality tested in Epithelial Cell Medium to ensure optimal morphology and growth over a period of at least 5 population doublings. Cervical Epithelial Cells are not exposed to antimicrobials or phenol red when cultured in Epithelial Cell Medium. Gentaur offers antimicrobials and phenol red, but they are not required for eukaryotic cell proliferation.
ABC-TC5507
Rat Primary Bladder Epithelial Cells from Gentaur are isolated from bladder tissue of 6-8 week old laboratory Sprague–Dawley rat. Rat Primary Bladder Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 0.5x106 cells and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.