Catalog number: 500 - SC066-LR
Product Category: Business & Industrial > Science & Laboratory
Size: 2 x 106 cell/ml x 1ml
SC066-LR
Luciferase & RFP expression stable cell line in Human ES2 cells with Puromycin resistance
SC066-R
RFP expression stable cell line in Human ES2 cells with Puromycin resistance
SC066-LG
Luciferase & GFP expression stable cell line in Human ES2 cells with Puromycin resistance
ABC-FC0042
HLiStCs are initiated by hepatic artery perfusion digestion with collagenase. HLiStCs are separated from the mixture of hepatic sinusoidal MV endothelial cells and Kupffer cells in the digestion solution. RFP-HLiStCs are selected from Zeocin resistant HLiStCs after infected with RFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3). Stellate-Growth medium is recommended for cell culture and these cells have a minimum average population doubling capacity 8 when cultured following the detailed protocol described below.
ABC-FC0045
HNPCs are initiated by dissecting fetal brain tissue and digestion with collagenase. RFP expressing HNPCs (RFP-HNPCs) are selected from RFP expressing lentiviruses infected HNPCs. RFP-HNPCs are offered in frozen vial format (the cells are provided @ passage 2). RFP-HNPCs growth medium is recommended for cell culture and these cells have a minimum average population doubling capacity 6 when cultured following the detailed protocol described below.
ABC-FC0046
Human dopaminergic neuronal cells are isolated from fetal brain tissue obtained from agencies authorized to procure and distribute tissues for research. Dopaminergic neuronal cells are selected in tyrosine free medium supplemented with a mixture of growth factors. Actively growing population of cells are tested for tyrosine hydroxylase (TH) expression by immunocytochemistry. RFP-Expressing Human dopaminergic neuronal cells (RFP-HDaNCs) are selected by puromycin after HDaNCs are infected with lentiviruses expressing RFP. Cells are provided with more than 5x10[5] cells/ vial at passage 2. The cells are grown in regular tissue culture dishes and subcultured at a split ratio of 1 to 2 at confluence. We recommend not to use the cells beyond 4 passages. There may be a significant decrease in the number of tyrosine hydroxylase positive cells after 4 passages as they are not maintained in selective medium to allow the growth of only cells expressing tyrosine hydroxylase.