Catalog number: 994 - ALHE21
Product Category: Business & Industrial > Science & Laboratory
Size: 1ml frozen Vial
ABC-TC3513
The walls of capillaries are composed of a single layer of microvascular endothe-lial cells. These cells differ in morphology and other properties depending on the tissues the capillaries supply. Therefore, Gentaur offers a range of Microvascular Endothelial Cells produced at Gentaur's cell culture facility from normal human tissues of different origins.Shortly after isolation, all Gentaur Microvascular Endothelial Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.
ABC-TC4081
Rat Bladder Microvascular Endothelial Cells from Gentaur are isolated from tissue of 7-day-old neonatal Sprague-Dawley Rats. Rat Bladder Microvascular Endothelial Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.Rat Bladder Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.