Pre-made lentiviral particles bicistronically express (under EF1a promoter) nuclear permeable CRE recombinase and the GFP marker under the same suEF1a promoter. A Puromycin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.
Pre-made lentiviral particles bicistronically express (under EF1a promoter) nuclear permeable CRE recombinase and the GFP marker under the same suCMV promoter. A Neomycin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.
Pre-made lentivirus express mitochondrial targeted pH-sensitive probe, Mito-SypHer under EF1a promoter. This lentivirus also contain the Blasticidin selection marker, provided in DMEM medium with 60ug/ml of polybrene.