Pre-made lentiviral particles bicistronically express (under EF1a promoter) nuclear permeable CRE recombinase and the GFP marker under the same suEF1a promoter. A Puromycin marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.
Integrase-Deficient Lentivirus expressing eGFP under EF1a promoter with Puromycin selection marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Pre-made lentivirus express GFP reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Pre-made lentivirus express GFP reporter under the human ICAM-2's promoter which expressed specifically in vasculature endothelial cells and megakaryocytes. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Pre-made lentivirus express GFP reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.