Pre-made lentiviral particles bicistronically express (under EF1a promoter) nuclear permeable CRE recombinase and the GFP marker under the same suEF1a promoter. A Puromycin marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.
Integrase-Deficient Lentivirus expressing eGFP under EF1a promoter with Puromycin selection marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Pre-made lentivirus express GFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.