GWB-PS79B7-500RXN - Notch Reporter Assay Kit (cAMP/PKA Pathway)
1444.81 €
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Catalog number: 247 - GWB-PS79B7-500RXN
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 500
79324
RARγ Reporter Cellular Assay Pack
The RARγ Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of retinoic acid receptor gamma (RARγ). The pack contains the RARγ Reporter (Luc)-HEK293 Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (GenBank Accession No. P13631-1). This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid, and the expression of RARγ is confirmed by Western blotting._x000D_Additionally, the pack includes cell culture medium (Thaw Medium 6) that has been optimized for use with HEK293 cells. Thaw Medium 6 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required.
3257.25 €
79379
CSF1R / SRE Reporter Kit (MAPK/ERK Signaling Pathway)
The CSF1R SRE Reporter Kit is designed for monitoring the activity of the CSF1R signaling pathway in cultured cells. The kit contains a transfection-ready vector for CSF1R and SRE luciferase reporter vector. Upon ligand binding, active CSF1R will initiate the MAPK/ERK signaling pathway, leading to expression of the SRE-controlled reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency._x000D_The kit also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity.
1083.22 €
60611
CRE/CREB Reporter Kit (cAMP/PKA Signaling Pathway)
The CRE/CREB Reporter kit is designed for monitoring the activity of the cAMP/PKA signaling pathway in cultured cells. The kit contains transfection-ready CRE luciferase reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response element (CRE) located upstream of a minimal promoter. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase. The CRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with the constitutivelyexpressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.