GFP and Luciferase Expressing Human Neonatal Dermal Fibroblasts
1336.38 €
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Catalog number: 535 - cAP-0008-neoGFP-Luc
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 1Frozen Vial
ABC-FC0027
GFP and Luciferase Expressing Human Neonatal Dermal Fibroblasts
HNDFCs-Neo cells are isolated from normal neonatal foreskin tissue samples and transfected with GFP and Luciferase expressing Lentiviral particles at passage one. Puromycin resistant GFP-Luc-HNDFCs-Neo are selected and shipped in frozen vial (the cell are provided @passage 3). Fibroblast Growth medium is recommended for cell culture and these cells have an average minimum population doubling levels 14 when cultured following the detailed protocol described below.
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ABC-TC3068
BALB/c Mouse Dermal Fibroblasts
BALB/c Mouse Primary Dermal Fibroblasts from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Dermal Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Primary Dermal Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.