Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under enhanced EF1a promoter with Neomycin antibioic marker. Particles was pellet down and re-suspended in PBS solution.
Pre-made lentiviral particles having RFP reporter under heat inducible Hsp70 promoter. This lentivirus also contain the Neomycin selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Negative control lentivirus contains a null spacer insert under EF1a promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the Neomycin marker under RSV promoter.
Lentivirus express SEAP reporter under a minimal promoter embded 8 tandem repeats of Tcf (Wnt responsive element). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.