EcoT38 I -HC unit: 900, High Concentration
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quantity
Produktdetaljer
Katalognummer: 377 - YRECOT38-HC
Produktkategori: Företag och industri > Vetenskap och laboratorium
Gentaur
Storlek: 1 vial
354263
CORNING® MATRIGEL® MATRIX HIGH CONCENTRATION (HC), GROWTH FACTOR REDUCED *LDEV-FREE, 10ML
Advanced Cells - DL; ECM - DL
1507.2 €
K088
asCpf1 Nuclease NLS Protein (High Concentration)
Using Cpf1 (a.k.a. Cas12a) in your CRISPR experiment offers several advantages over other CRISPR-associated nucleases.;<ul><li>Due to the T-rich PAM sequence (TTTN), Cpf1 enables editing in regions unable to be targeted by Cas9. </li><li>Cpf1 can be used with a shorter guide RNA (called crRNA) than Cas9. </li><li>Cpf1 creates a staggered cut in dsDNA instead of a blunt cut. </li><li>Cpf1 cuts distal to the PAM sequence, which may allow for multiple rounds of cleavage. </li><ul>asCpf1 is from the bacteria <i>Acidaminococcus</i>. This protein contains a SV40 T antigen nuclear localization signal (NLS) on the N-terminus of the protein. If the cut caused by asCpf1 is repaired by non-homologous end joining (NHEJ), an indel may be formed that disrupts the open reading frame of the targeted gene, leading to gene knockout. Alternatively, by supplying a repair template, a sequence can be knocked in at the cleavage site via homology directed repair (HDR).
378.5 €
K089
saCas9 Nuclease NLS Protein (High Concentration)
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;;The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).;;The Cas9 nuclease from the bacteria <i>Staphylococcus aureus</i>, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5’-NNGRRN (preferably 5’-NNGRRT). saCas9 Nuclease NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.