Diablo:XIAP Inhibitor Screening Assay Kit

LKU606061-96T

Diablo Homolog (DIABLO) Magnetic Luminex Assay Kit

759.6 €

LKU606084-96T

Diablo Homolog (DIABLO) Magnetic Luminex Assay Kit

809.28 €

32521

BRD1 Inhibitor Screening Assay Kit

The BRD1 Inhibitor Screening Assay Kit is designed to measure the inhibition of BRD1 binding to its substrate. The key to the BRD1 Inhibitor Screening Assay Kit is the specific binding of the BRD1 bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRD1 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

915 €

82210

COX2 Inhibitor Screening Assay Kit

The COX2 Inhibitor Screening Assay Kit is designed to measure COX2 (cyclooxygenase 2) activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough purified recombinant COX2, Amplex™ Red, Arachidonic Diluent, COX Assay Buffer, and 100% ethanol for 100 enzyme reactions.

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82726-1

USP9X Inhibitor Screening Assay Kit

The USP9X Inhibitor Screening Assay Kit is a fluorogenic assay kit designed to measure the activity of the deubiquitinating (DUB) enzyme USP9X (ubiquitin specific peptidase 9 X-linked) for screenin82726-1g and profiling applications. The kit contains enough purified USP9X, Ubiquitinated-AMC substrate, and assay buffer for 96 reactions.To determine the effect of an inhibitor on USP9X activity, the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of the DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteases.Figure 1: Illustration of the assay principle.Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases, with a C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC) . In the conjugated form, the energy emitted from fluorochrome AMC is quenched. Upon proteolysis, AMC is no longer quenched and emits fluorescence with an λexcitation/λemission maxima of 350/460 nm. The increase in fluorescence is proportional to the DUB activity.Need us to run inhibitor screens or profile your compounds against USP14? Check out ourProtease Screening ServicesorUbiquitination Screening Services.

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82742

USP22 Inhibitor Screening Assay Kit

The USP22 Inhibitor Screening Assay Kit is a fluorogenic assay kit designed to measure the activity of the deubiquitinating (DUB) enzyme USP22 (ubiquitin specific peptidase 22) for screening and profiling applications. The kit contains enough purified USP22, Ubiquitinated-AMC substrate, and assay buffer for 100 reactions.To determine the effect of an inhibitor on USP22 activity, the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of the DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteases.Figure 1: Illustration of the assay principle.Ubiquitin-AMC (Ub-AMC) is a fluorogenic substrate for ubiquitin hydrolases, based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC) . In the conjugated form the energy emitted from the fluorochrome AMC is quenched. Upon release from Ub, AMC emissions are no longer quenched fluorescence with λexcitation/λemission maxima of 350/460 nm is emitted. The increase in fluorescence is proportional to the DUB activity.Need us to run inhibitor screens or profile your compounds against USP22? Check out ourProtease Screening ServicesorUbiquitination Screening Services.

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