Recombinant Dengue Virus serotype 2 virus-like particle recombinant antigen consisting of Envelope, pre-Membrane and Membrane protein produced in 293 human cells. The C-terminal 20% of the dengue Envelope protein was replaced by the corresponding Japanese encephalitis virus SA-14 sequence (Envelope protein amino acids 397-495). The replaced sequence corresponds to the transmembrane and intraparticle portion of the protein.