Recombinant HEK293 cells constitutively expressing human GPRC5D (the orphan G protein-coupled receptor, class C group 5 member D, NM_018654.1) under the control of a CMV promoter.
Recombinant HEK293 cell line expressing human PDE1B (phosphodiesterase 1B, accession number NM_000924). ; N-terminal His-tagged human PDE1B has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The function of PDE1B was characterized by a cell-based cGMP assay. In this assay system, cells co-express atrial natriuretic peptide (ANP) receptor A (a guanylate cyclase), cyclic nucleotide5gated cation channel CNGA2, and the photoprotein aequorin. Intracellular cGMP level is monitored via aequorin luminescence induced by Ca<sup>2+</sup> influx through CNGA2, acting as the intracellular cGMP sensor.