GPRC5D HEK293 Cell Line
14392.50 €
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Catalog number: 421 - 78345
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 2 vials
78374
FcRL5 HEK293 Cell Line
Recombinant HEK293 cells constitutively expressing full length human FcRL5 (Fc Receptor Like 5, also known as CD307, ref. seq. NM_031281).
10983.75 €
60402
PDE1B-HEK293 Cell line
Recombinant HEK293 cell line expressing human PDE1B (phosphodiesterase 1B, accession number NM_000924). ; N-terminal His-tagged human PDE1B has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The function of PDE1B was characterized by a cell-based cGMP assay. In this assay system, cells co-express atrial natriuretic peptide (ANP) receptor A (a guanylate cyclase), cyclic nucleotide5gated cation channel CNGA2, and the photoprotein aequorin. Intracellular cGMP level is monitored via aequorin luminescence induced by Ca<sup>2+</sup> influx through CNGA2, acting as the intracellular cGMP sensor.
14195.55 €
60407
PDE7A-HEK293 Cell Line
Recombinant HEK293 cell line expressing human PDE7A (phosphodiesterase 7A, accession number NM_002603). ; N-terminal FLAG-tagged human PDE7A has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE7A expression was confirmed by Western blotting. The regulation of intracellular level of cAMP by PDE7A in PDE7A stably-expressed HEK293 cells was characterized by a cell-based reporter assay using pCRE-luc reporter vector. pCRE-luc contains a luciferase gene that is under the control of the cAMP response element (CRE). When cells transiently transfected with pCRE-luc reporter were activated by forskolin, the level of cAMP was upregulated in parental HEK293 cells, inducing the expression of the luciferase reporter, whereas hPDE7A-HEK293 cells showed reduction in the level of cAMP that resulted in lowered expression of luciferase. Inhibition of PDE7A activity by BRL 50481, a PDE7A inhibitor, restored the cAMP level, resulting in higher luciferase activity.
14195.55 €