Human IL-2 reporter construct is stably integrated into the genome of Jurkat T-cells. The firefly luciferase gene is controlled by a human IL-2 promoter.
Transformed from human Jurkat T lymphocyte suspension cells using lentivirus expressing firefly luciferase gene. The Luciferase was expressed under the enhanced EF1a promoter. The Puromycin marker was expressed under Rsv promoter. The cell line demonstrates high level of bioluminescence signal via firefly luciferase assay.