C57BL/6 Mouse Primary Bladder Epithelial Cells from Gentaur are isolated from bladder tissue of pathogen-free laboratory mice. C57BL/6 Mouse Primary Bladder Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaurandrsquo; Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5×106 cells per ml and is delivered frozen.