ABC-TC5519

Human Kidney Fibroblasts

Human Kidney Fibroblasts are isolated from the human adult healthy renal tissue. HKF-a are cryopreserved after passage one culture and delivered frozen. HKF-a are guaranteed to further expand for 15 population doublings under the conditions provided by in the technical sheet.As an excretory organ in the urinary system, the kidney greatly influences an individual’s well being by preserving homeostasis through regulating electrolytes, pH, and blood pressure among other control mechanisms. Comprising the majority of interstitial cells in the kidney, renal fibroblasts are responsible for the synthesis of collagen I of the extracellular matrix. Also, renal cortical fibroblasts synthesize erythropoietin, an important glycoprotein hormone that controls the production of red blood cells and renal medullary fibroblasts produce prostaglandins, a class of autocrine or paracrine hormones, which help maintain water and electrolyte homeostasis. Renal interstitial fibrosis, a disorder that may escalate to chronic kidney disease, is often correlated with excessive deposition of extracellular matrix by renal fibroblasts, the regulation of which process is under intensive research.

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ABC-TC4147

Rat Kidney Fibroblasts

Rat kidney fibroblasts are isolated from tissue of 1-day-old neonatal Sprague–Dawley rats. Rat kidney fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.

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CSC-8240W

Human Kidney Fibroblasts

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ABC-TC3064

BALB/c Mouse Cardiac Fibroblasts

BALB/c Mouse Primary Cardiac Fibroblasts from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Cardiac Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Primary Cardiac Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

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ABC-TC3068

BALB/c Mouse Dermal Fibroblasts

BALB/c Mouse Primary Dermal Fibroblasts from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Dermal Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Primary Dermal Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

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ABC-TC3070

BALB/c Mouse Embryonic Fibroblasts

Mouse Embryonic Fibroblasts from are isolated from day 13 mouse embryos of pathogen-free laboratory mice. They are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium for 3-7 days.

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