Catalog number: 420 - 8620829
Product Category: Business & Industrial > Science & Laboratory
Size: 1EA
ABC-TC3134
Bovine large artery smooth muscle cells are derived from tunica intima and tunica media of healthy, fibrous, plaque-free bovine large arteries. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings.
ABC-TC3234
C57BL/6 Mouse Primary Pulmonary Artery Fibroblasts from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Pulmonary Artery Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Primary Pulmonary Artery Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
ABC-TC3267
Canine Pulmonary Artery Smooth Muscle Cells (CnPASMC) are derived from the tunica intima and tunica media of canine pulmonary arteries and are cryopreserved at second passage. CnAOEC can be passaged at least 16 population doublings.