TIGIT / NFAT Reporter - Jurkat Cell Line
16596.83 €
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product details
Catalog number: 421 - 60538
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 2 vials
60621
NFAT Reporter (Luc) - Jurkat Cell Line
The NFAT Reporter - Jurkat Cell Line contains a firefly luciferase gene under the control of the_x000D_NFAT response element stably integrated into Jurkat cells. This cell line has been validated for_x000D_response to thapsigargin, ionomycin, and phorbol 12-myristate 13-acetate (PMA). It is useful as_x000D_a control cell line for other NFAT reporter cell lines expressing various immune checkpoint_x000D_receptors.
3628.43 €
78323
TMIGD2/NFAT Luciferase Reporter Jurkat Cell Line
Recombinant Jurkat cell line expressing firefly luciferase under the control of an NFAT response element, and with constitutive expression of human TMIGD2 (Transmembrane and immunoglobulin domain containing 2; CD28H; NM_144615). Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity.
15816.60 €
78384
NFAT Reporter (eGFP) Jurkat Recombinant Cell Line
Recombinant Jurkat T-cells expressing an enhanced GFP (eGFP) gene under the control of NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway can be monitored by examining eGFP expression.
3545.10 €
79853
Anti-CD19 CAR / NFAT (Luciferase) Reporter Jurkat Cell Line (CD19 SCFV-CD28-4-1BB-CD3ζ)
Anti-CD19 CAR/NFAT-luciferase reporter Jurkat cell line is a double stable cell line expressing anti-CD19 CAR and NFAT-luciferase reporter. It is made from the anti-CD19 CAR lentivirus (BPS Bioscience #79851). The reporter cell line has been validated for anti CD19-CAR expression by FACS, and for luciferase reporter activation stimulated by target cells including CD19/CHO recombinant cell line can be used for primary screening and functional validation of anti-CD19 CAR construct and lentivirus before testing in primary T cells.<br /><br />Anti-CD19 CAR consists of anti-CD19 scFv linked to 3rd generation CAR (Chimeric Antigen Receptor) containing CD28, 4-1BB co-stimulatory domains, and CD3ζ signaling domain.<br />
15816.60 €
78322
KIR3DL3/IL-2 Luciferase Reporter Jurkat Cell Line
Recombinant Jurkat cell line expressing firefly luciferase under the control of an IL-2-responsive promoter, and with constitutive expression of human KIR3DL3 (Killer Cell Immunoglobulin Like Receptor, Three Ig Domains and Long Cytoplasmic Tail 3; GenBank accession #BC143802.1 corresponding to KIR3DL3*00402 allele). HHLA2 (B7-H7) mediates an immune-stimulatory signal via TMIGD2 (Transmembrane and immunoglobulin domain containing 2; CD28H) in naïve T cells while it delivers an immune-inhibitory signal through KIR3DL3 in activated T cells and Natural Killer (NK) cells.
16596.83 €
79310
HVEM/NF-κB Reporter Jurkat Recombinant Cell Line
Recombinant clonal stable Jurkat T cell line expressing firefly luciferase gene under the control of 4 copies of NF-κB response elements with constitutive expression of human HVEM (Herpes Virus Entry Mediator, Tumor Necrosis Factor Receptor Superfamily Member 14, TNFRSF14, CD270, GenBank Accession No. NM_003820). Following activation by human HVEM ligand LIGHT, NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase reporter gene._x000D_Materials Required but Not Supplied: _x000D_Assay Medium: Thaw Medium 2 (BPS Cat. #60184)LIGHT, His-Tag (Human) (BPS Cat. #71266LIGHT-CHO Recombinant Cell Line (BPS Cat. # 79262)96-well tissue culture-treated white clear-bottom assay plateOne-Step luciferase assay system (BPS bioscience # 60690) or other luciferase reagents for measuring firefly luciferase activityLuminometer