Assay #1: Measured by its ability to induce alkaline phosphatase production by ATDC-5 Cells, The ED50 for this effect is typically 0.07-0.2 µg/mL.
Assay #2: Measured by its ability to induce alkaline phosphatase production by C2C12 cells, The ED50 for this effect is typically 0.2-1 µg/mL.