Rabbit polyclonal anti-GAPDH antibody, Loading control
Ask for quotation
On request
quantity
product details
Catalog number: 937 - TA890003
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 100 µl
BS65529
GAPDH (Loading Control) polyclonal antibody
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
361.58 €
BS65656
GAPDH (Loading Control) Polyclonal Antibody
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
361.58 €
PSI-1832-002mg
Loading Control Detection Set Alpha-Tubulin, GAPDH
Loading controls are used in immunoblots to normalize the levels of protein detected by confirming that protein loading is the same across the gel. The expression levels of the loading control should not vary between the different sample lanes. Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample. This ensures constant expression levels. Thus “housekeeping genes” such as Beta-actin, Alpha-tubulin, and GAPDH are frequently chosen for use as loading controls. Actin is a ubiquitous protein and a major component of the eukaryotic cytoskeleton. Globular tubulin subunits made up of alpha- and beta-tubulin heterodimers are the building blocks of microtubules, one of three types of cytosolic fibers that comprise the cytoskeleton. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism.