Immunofluorescence staining of Hela cells with CSB-RA008968A2481phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
A polyclonal antibody raised in Rabbit that recognizes and binds to Human mTOR (FRAP1) (S2481). This antibody is tested and proven to work in the following applications: