The OX40[Biotinylated]:OX40L Inhibitor Screening Assay Kit is designed for_x000D_screening and profiling inhibitors of OX40:OX40L interaction. The key to this kit is the high sensitivity of detection of biotinlabeled_x000D_OX40 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for_x000D_the assay. First, OX40L is coated on a 96-well plate. Next, OX40-biotin is incubated with OX40L_x000D_on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP_x000D_substrate to produce chemiluminescence, which can then be measured using a_x000D_chemiluminescence reader.