Catalog number: 10021 - T6488
Product Category: Business & Industrial > Science & Laboratory
Size: 1x10^6 cells / 1.0 ml
SC033
Inducible GFP expression stable cell line in HEK293 cells, with Blasticidin and puromycin resitance
78581
Recombinant stable HEK293 cell line constitutively expressing RFP (Red Fluorescent Protein) and GFP (Green Fluorescent Protein), which have been stably integrated into the AAVS1 safe harbor locus on chromosome 19._x000D_Expression of RFP is driven by a CMV promoter, whereas GFP is driven by an EF1A promoter. The combined DNA fragment (CMV-RFP-bGH Poly A-EF1A-GFP-T2A-Puro-SV40 Poly A) (Figure 1) was integrated at the AAVS1 safe harbor locus using CRISPR/Cas9 technology. Cells were cloned by limiting dilution to obtain a monoclonal population. Without the adverse effects resulting from random integrations of RFP-GFP into the HEK293 genome, this cell line behaves like parental HEK293 cells.
60402
Recombinant HEK293 cell line expressing human PDE1B (phosphodiesterase 1B, accession number NM_000924). ; N-terminal His-tagged human PDE1B has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The function of PDE1B was characterized by a cell-based cGMP assay. In this assay system, cells co-express atrial natriuretic peptide (ANP) receptor A (a guanylate cyclase), cyclic nucleotide5gated cation channel CNGA2, and the photoprotein aequorin. Intracellular cGMP level is monitored via aequorin luminescence induced by Ca<sup>2+</sup> influx through CNGA2, acting as the intracellular cGMP sensor.