Compared to fluorescein labeling reagents such as FITC and FAM, 5-dR110 [5-carboxy-4,7-dichlororhodamine 110] reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength.
Immobilized metal affinity chromatography (IMAC) is a popular method for protein purification, particularly for recombinant proteins fused to a polyhistidine-tag.