Membrane-Protein---Human-Adult-Normal-Tissue:-Spinal-Cord
871.41 €
871.41 €
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product details
Catalog number: 36 - P3234234
Product Category: Business & Industrial > Science & Laboratory
Supplier:
Gentaur
Size: 0.1 mg
1377
Spinal cord Lysate
Spinal cord tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl<sub>2</sub>, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
478.54 €