Recombinant IL-11 is a disulfide-linked monomer protein consisting of 179 amino acid residues, and migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Interleukin-11 mature chain was expressed in E. coli.
Recombinant IL-11 is a disulfide-linked monomer protein consisting of 179 amino acid residues, and migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Interleukin-11 mature chain was expressed in E. coli.
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of murine B9-11 was found to be less than 1 ng/ml, corresponding to a specific activity of >1 x 107 IU/mg.