HUVECs were infected with Lentiviral particles expressing GFP-Tagged Cytochrome C oxidase VIII @ passage 1. Puromycin resistant Mitochondria-GFP-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below).
Pre-screened for endothelial properties. Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from normal human umbilical vein. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doubling.