Human Lymph PrimaCell™3: Normal Lymphatic Fibroblasts Growth Medium
614.85 EUR
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Produktdetaljer
Katalognummer: 567 - 9-46089
Produktkategori: Företag och industri > Vetenskap och laboratorium
Gentaur
Storlek: 5 x 100 ml
ABC-TC3216
C57BL/6 Mouse Lymphatic Fibroblasts
C57BL/6 Mouse Primary Lymphatic Fibroblasts from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Lymphatic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Primary Lymphatic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
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