9-80273

Rat Lung Tissue Preparation Buffer 3: Normal Pulmonary Artery Fibroblasts

498.20 €

9-80181

Mouse Lung Tissue Preparation Buffer 4: Normal Pulmonary Artery Fibroblasts

498.20 €

9-80180

Mouse Lung Tissue Preparation Buffer 3: Normal Pulmonary Artery Endothelial Cells

425.19 €

ABC-TC4202

Rat Pulmonary Artery Fibroblasts

Rat Primary Pulmonary Artery Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Pulmonary Artery Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Pulmonary Artery Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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9-80111

Human Ureter Tissue Preparation Buffer 1: Normal Lliac Artery Endothelial Cells

425.19 €

9-80173

Mouse Kidney Tissue Preparation Buffer 4: Normal Renal Artery Endothelial Cells

425.19 €