K030

Cas9 Nuclease NLS Protein

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;;The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).;;The Cas9 nuclease from the bacteria <i>Streptococcus pyogenes</i>, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First the spCas9 PAM sequence is 5’-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency <i>in vivo</i> compared to other variants. Cas9 Nuclease NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.

329.26 €

MBS4156498-0008mg

Cas9 Nuclease NLS Protein

444.40 €

MBS4156498-5x0008mg

Cas9 Nuclease NLS Protein

953.06 €

K187

fnCpf1 Nuclease NLS Protein (High Concentration)

Using Cpf1 (a.k.a. Cas12a) in your CRISPR experiment offers several advantages over other CRISPR-associated nucleases.;<ul><li>Due to the T-rich PAM sequence (TTTN), Cpf1 enables editing in regions unable to be targeted by Cas9. </li><li>Cpf1 can be used with a shorter guide RNA (called crRNA) than Cas9. </li><li>Cpf1 creates a staggered cut in dsDNA instead of a blunt cut. </li><li>Cpf1 cuts distal to the PAM sequence, which may allow for multiple rounds of cleavage. </li><ul>fnCpf1 is from the bacteria <i>Francisella novicida</i>. This protein contains a SV40 T antigen nuclear localization signal (NLS) on the N-terminus of the protein. If the cut caused by fnCpf1 is repaired by non-homologous end joining (NHEJ), an indel may be formed that disrupts the open reading frame of the targeted gene, leading to gene knockout. Alternatively, by supplying a repair template, a sequence can be knocked in at the cleavage site via homology directed repair (HDR).

670.13 €

CAA-L5140

GENPower™ NLS-Cas12a Nuclease (MALS verified)

GENPower™ NLS-Cas12a Nuclease (CAA-L5140) is expressed from E. coli cells. It contains AA Ser 2 - His 1228 (Accession # A0A5S8WF58).

541.80 €

CAA-L5140-100ug

GENPower™ NLS-Cas12a Nuclease (MALS verified)

GENPower™ NLS-Cas12a Nuclease (CAA-L5140) is expressed from E. coli cells. It contains AA Ser 2 - His 1228 (Accession # A0A5S8WF58).

541.80 €