A fluorogenic, acetylated peptide substrate for class I HDACs (HDACs 1, 2, and 3) and class 2b HDACs (HDACs 6 and 10). This substrate fluoresces at excitation 350-380 nm, emission at 440-460 nm. If autofluorescence of the inhibitor near this wavelength is an issue, we recommend using Fluorogenic HDAC substrate 2 (green), BPS catalog #50038. For class 2a HDACs (HDACs 4, 5, 7, and 9) or HDACs 8 and 11, we recommend #50040.