Fetal Spinal Cord Lysate
888.95 €
888.95 €
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product details
Catalog number: 223 - XBL-10130
Product Category: Business & Industrial > Science & Laboratory
Supplier:
Gentaur
Size: 0.1 mg
1377
Spinal cord Lysate
Spinal cord tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl<sub>2</sub>, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
478.54 €
Fetal human spinal Cord tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human spinal Cord tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the spinal Cord tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The spinal Cord tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.