Mouse HDAC1, GenBank Accession No. NM_008228, amino acids 1-482 (full length), with C-terminal His-tag and C-terminal FLAG-tag, MW = 57 kDa, expressed in an Sf9 baculovirus expression system. Recombinant HDAC1 forms a complex with endogenous Hsp70. The identity of Hsp70 was confirmed by MALDI-TOF mass spectrometry.
Mouse CD70, also known as CD70 antigen, Tumor Necrosis Factor Ligand Superfamily Member 7, TNFSF7, CD27 Ligand, Ki-24 antigen, CD27-L, and CD27LG, GenBank Accession No. NM_011617, a.a. 45-195 with N-terminal His-tag, expressed in a HEK293 cell expression system. MW = 19 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.