The eIF4B (Phospho-Ser422) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor eIF4B (Phospho-Ser422) protein expression profile in cells. The kit can be used for measuring the relative amounts of eIF4B (Phospho-Ser422) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on eIF4B (Phospho-Ser422).