4252-040-01

CometAssay HT Slide

410.38 €

4252-500-01

CometAssay HT Slide

1189.21 €

MIC4066

Slide Box 25 Slide Polystyrene - EACH

135.58 €

MIC4052

Slide Mailer PP Hinged Lid 5 Slide - EACH

112.86 €

MIC4054

Slide Mailer Polythene Hinged Lid 2 Slide - PK100

223.59 €

1218

HT-29 Lysate

HT-29 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The HT-29 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

478.54 €