BKS DB Mouse Dermal Microvascular Endothelial Cells
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Catalog number: 870 - ABC-TC5480
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 1 vial
ABC-TC5480
BKS DB Mouse Dermal Microvascular Endothelial Cells
BKS db Mouse Dermal Microvascular Endothelial Cells are isolated from the dermal microvascular of Mice homozygous for the diabetes spontaneous mutation (Lepr/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic. BKS db Mouse Dermal Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
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