Acetyl-CoA Assay Kit
841.84 €
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Catalog number: 639 - abx294402-20g
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 20 µg
79558
PDE4B1 Assay Kit
Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4 selective inhibitors are currently in clinical trials for the treatment of diseases related to inflammatory disorders. PDE4B1 isoform expression predominates in cortex, however lower expression of PDE4B1 has been observed in the cerebella of subjects with autism when compared with matched controls. The PDE4B1 Assay Kit is designed for identification of inhibitors of PDE4B1 using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE4B1 to the binding agent._x000D_Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light._x000D_The PDE4B1 Assay Kit comes in a convenient 96-well format, with purified PDE4B1 enzyme, fluorescently labeled PDE4B1 substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE4B1 Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4B1 reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE4B1 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.
795.38 €
50293
HSP90α Assay Kit
The HSP90α Assay Kit is designed for identification of HSP90α inhibitors using fluorescence polarization. The assay is based on the competition of fluorescently labeled geldanamycin for binding to purified recombinant HSP90α. The key to the HSP90α Assay Kit is the fluorescently labeled geldanamycin. Using this kit, only one simple step on a microtiter plate is required for HSP90α reactions. The fluorescently labeled geldanamycin is incubated with a sample containing HSP90α enzyme to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.