15mL Peripheral Blood Lymphocyte Separation Tube, 50/pk, 150/cs
356.81 €
In Stock
quantity
product details
Catalog number: 1007 - 601851
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 150 pcs/cs
601852
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50mL Peripheral Blood Lymphocyte Separation Tube,25/pk, 500/cs
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Peripheral Blood Leukocyte (PBL's) Lysate
Peripheral Blood Leukocyte (PBL's) tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl<sub>2</sub>, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.