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IM9701
Spoligotyping Kit with Primers and Controls
<p> A very simple, inexpensive and effective tool for Tuberculosis/ Mycobacterium research. Spoligotyping is a PCR-based Method to Simultaneously Detect and Type <i> Mycobacterium Tuberculosis </i> Complex Bacteria. Spoligotyping, which uses RLB (Reversed Line Blotting) offers an alternative for typical Southern blotting when rapid results are required. The method is particularly useful to simultaneously detect and type <i>M. tuberculosis</i> complex bacteria in clinical samples (suspected nosocomial infections, outbreaks in prisons, etc.). The level of differentiation by spoligotyping is less compared to IS6110 fingerprinting for strains having five or more IS6110 copies, but higher for strains with less than five copies. Thus, Spoligotyping is a preferred method to type <i>M. bovis</i> strains, which usually contain only one or two IS6110 copies. Note, that <i>Mycobacterium bovis</i> can be recognized by the absence of reactivity with spacers 39-43.</p>
2637.11 €
IM9702
Spoligotyping Membrane
<p> A very simple, inexpensive and effective tool for Tuberculosis/ Mycobacterium research. Spoligotyping is a PCR-based Method to Simultaneously Detect and Type <i> Mycobacterium Tuberculosis </i> Complex Bacteria. Spoligotyping, which uses RLB (Reversed Line Blotting) offers an alternative for typical Southern blotting when rapid results are required. The method is particularly useful to simultaneously detect and type <i>M. tuberculosis</i> complex bacteria in clinical samples (suspected nosocomial infections, outbreaks in prisons, etc.). The level of differentiation by spoligotyping is less compared to IS6110 fingerprinting for strains having five or more IS6110 copies, but higher for strains with less than five copies. Thus, Spoligotyping is a preferred method to type <i>M. bovis</i> strains, which usually contain only one or two IS6110 copies. Note, that <i>Mycobacterium bovis</i> can be recognized by the absence of reactivity with spacers 39-43.</p>
2509.85 €
MN45
Mini-blotter for Spoligotyping
This is mini-blotter unit only. Spoligotyping Kit with primers and controls IM9701 is not included.
5982.23 €
PC200
Foam Cushions
<p>Foam Cushions (PC200) are intended for use with MiniblotterTM instruments. The MiniblotterTM instrumentsenable you to perform multiple antibody or probe incubations on a single membrane, using microliter volumes ofreagent. Typical applications include monoclonal antibody screening on western blots, animal and humanscreening, and DNA probe hybridizations. Sample processing is streamlined through use of the washingmanifold, which enables washing of all sample lanes, simultaneously. Reactions can be detected by allconventional methods, including enzyme conjugates, chemiluminescence and radiolabels.</p>